High-performance liquid chromatography, HPLC for short, is a type of liquid chromatography that is used to separate compounds from dissolved solutions. This test is aimed at separating and identifying the constituent components of any dissolved compound.
The testing technique finds various uses in a variety of industries, such as pharmaceutical, polymer, coatings, and medical among other commercial industries. This testing technique is mandatory for many industries which are governed by strict industry standards and regulations.
Choosing an HPLC course from leading technical institutes in India is helpful to create a fruitful career as an analytical chemist. This course will teach you everything that you need to learn in testing raw materials and finished products.
With your chemical analysis skills, your employer will be able to test the raw material of any new material supplier immediately and can also get quality assurance tests performed frequently. This is an extremely cost-efficient way to ensure that the industry produces top quality products at all times.
What is the importance of the HPLC Course in 2020?
Product manufacturers will tell you that many raw materials look and feel alike but they may have vastly different components that can change their properties and features drastically. Hence, there is a constant need for frequently testing and verifying raw materials before using them in the production stage to ensure the quality of the product.
Manufacturers also need HPLC trained professionals and laboratories to reverse engineer formulations, perform competitor product analysis, resolve product failure problems, as well as to detect any impurities of deformities in the raw material or finished product.
Frequently, the manufacturer needs HPLC to ensure constant quality checks for finished goods to ensure that the amount of ingredients quoted on the product label is accurate. This is also a better and more efficient way to check and identify defects or contaminants which may develop during manufacturing.
This way, the manufacturer can decrease the chances of having to recall defective products once they are out in the distribution and sales stages. Nowadays, industries require performing HPLC tests along with other related testing techniques to ensure that their products have a stable shelf life.
A great advantage of using the HPLC testing technique is that it only requires a small sample size for performing the relevant tests. The testing technique is flexible and can be enhanced to include additional quantitative parameters for producing reliable and accurate results.
Industries can benefit greatly from HPLC trained professionals and laboratories as this helps to improve the quality of their products, achieve a better understanding of the competitor’s products and avoid the risk of product recall due to undetected defects or contamination.
What makes High-Performance Liquid Chromatography (HPLC) better than other Chromatography Techniques?
The first major difference between other chromatography techniques and HPLC is that it uses a gas as the mobile phase and separates the components after vaporization. This way, it becomes possible to separate and identify the compounds having very small molecular weights in the mobile phase.
The HPLC technique requires the sample to be in either gas or liquid state. This is easier to vaporize in the injection port of the testing equipment. A carrier gas, usually helium, is used for the mobile phase in gas chromatography because of its low molecular weight and chemical inertness.
When the pressure is applied, the mobile phase moves the analyte through the chromatography column which is coated with a stationary phase that helps to separate the different components in the sample.
Leading HPLC course providers in India not only teach the essential and advanced study material but also allow ample on-site training for students to ensure that they are well-prepared to face the challenges in real-life gas chromatography.
What is the scope after the HPLC Course in India?
There is an immense scope after completing an HPLC course in India. The course prepares you for performing HPLC analysis which is frequently used in several major industries to determine the components of chemical products.
For instance, the HPLC tests are frequently used for assuring the quality of products in the chemical industry and for measuring the level of toxic substances in the air, water, and soil for adhering to pollution restricting regulations in the country.
So, aspiring analytical chemists need to choose the technical institute which offers a comprehensive course material along with adequate lab time to fine-tune the gas chromatography skills.